Detection of IL‑23 R in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes treated with 50 ng/mL PMA, 200 ng/mL Calcium Ionomycin, 20 ng/mL Recombinant Mouse IL‑23 (), 40 ng/mL Recombinant Mouse IL‑6 (), and 200 ng/mL LPS overnight to induce Th17 activation were stained with Rat Anti-Mouse CD3 APC‑conjugated Monoclonal Antibody () and either (A) Rat Anti-Mouse IL‑23 R PE‑conjugated Monoclonal Antibody or (B) Rat IgG1 Phycoerythrin Isotype Control.